2024 The dissolution of double holliday junctions

The dissolution of double holliday junctions

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WebOct 10, 2010 · Sgs1 and Top3, stimulated by replication protein A (RPA), dissolve double Holliday junctions to yield non-crossover products. (a) A schematic representation of the double Holliday junction substrate (DHJS) showing dissolution by convergent branch migration or resolution by nucleolytic cleavage.Dissolution of the dHJ leads exclusively to … WebCiteSeerX — The Dissolution of Double Holliday Junctions CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Telomeres have evolved to protect the ends of linear chromosomes from themyriad of threats posed by the cellular DNA damage signaling and repair pathways.

Roles of SLX1–SLX4, MUS81–EME1, and GEN1 in avoiding …

WebJul 1, 2014 · Holliday junctions (HJs) can be formed between sister chromatids or homologous chromosomes during the recombinational repair of DNA lesions. A variety of … WebParticipates in DNA replication and repair. Involved in 5"-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5"-ssDNA. Negatively regulates sister chromatid exchange (SCE). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. example of hypothetico-deductive reasoning

Holliday junction - Wikipedia

WebAll chains were made of 3 components: an AS1411 aptamer at the 3ʹ end, followed by a 5-nt poly-A linker, and the sequence that made up the Holliday junction at the 5ʹ end. The four strands were expected to form a cross-shaped nanostructure (Apt-HJ) in a self-assembled way, with each arm of the cross made up by a 17-bp double-stranded structure. WebIn this chapter, we provide a detailed experimental protocol for the preparation of an oligonucleotide-based, double Holliday junction substrate and for the biochemical analysis of dissolution in vitro. Based on techniques Marjana Tomic-Canic 1996, Springer Protocols WebJan 1, 2008 · The resultant double Holliday junction (dHJ) is a substrate either for dissolution into non-crossover products by BLM-TOPOIIIα or for resolution by a structure-specific endonuclease into ... bruno wrestling

Human Gene ATM (ENST00000278616.9) from GENCODE V43

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WebJul 1, 2014 · Coronavirus: Find the latest articles and preprints ... example of hypothesis with mediating variableWebApr 10, 2024 · Alternatively, some D-loop intermediates will become a double-Holliday junction (dHJ) if another end of the DSB is annealed to the displaced strand (i.e., second-end capture). dHJ can be dissolved through two pathways. First, a combination of helicase and topoisomerase dissolves dHJ from both ends, thus giving rise to non-crossovers … example of i-130 form filled out

"WebJun 30, 2005 · Double Holliday junction dissolution is specific for BLM. Despite the similar biochemical properties of many RecQ helicases in vitro, and their ability to act upon a wide variety of DNA structures, an elevated frequency of SCEs is thought to be a feature unique to BLM-deficient cells among human RecQ helicase mutants. " - The dissolution of double holliday junctions

The dissolution of double holliday junctions

Dissolution of Double Holliday Junctions by the Concerted Action …

WebJul 1, 2014 · DNA centric view showing three distinct pathways of Holliday junction processing. The BTR complex disengages dHJs, using the mechanism of “dissolution”, to generate NCO recombinants. MUS81-EME1 and SLX1-SLX4 interact to form the SLX-MUS complex which resolves both single and double HJs by endonucleolytic cleavage to … WebDissolution of double Holliday junctions by human BTRR complex. (A) Schematic diagram showing the dissolution of dHJ DNA to yield noncrossover products. ATP-dependent …

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WebSep 6, 2024 · Gene ID: 80010, updated on 15-May-2024 Summary RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double … WebMay 3, 2024 · HJs (double Holliday junctions [dHJs]) converge in an adenosine triphosphate (ATP)- hydrolysis – dep endent r eacti on to form a h emicat enan e that is su bseque ntly de caten ated by ...

WebJan 2, 2014 · Although Blm and Top3α are known to form a minimal dissolvasome that can uniquely undo a double Holliday junction structure, the details of the mechanism remain unknown. It was originally suggested that Blm acts first to create a hemicatenane structure from branch migration of the junctions, followed by Top3α performing strand passage to … WebKeywords: DNA repair, double-strand breaks, genome stability, homologous recombination, interstrand crosslinks, stalled replication forks Cell Research (2008) 18:99-113. doi: 10.1038/cr.2008.1; published online 1 January 2008 Introduction DNA damage is a fact of life as a consequence of endog-enous sources and processes as well as exogenous …

Webactivities include ATP-dependent Holliday junction migration, 3'-5' strand displacement, and ATP-independent single-strand annealing (9, 10). Most significantly, BLM protein in conjunction with topoisomerase IIIa (Top3a) can resolve double Holliday junctions (dHJs) via dissolution or convergent migration (11-14). WebApr 22, 2024 · Design of HR Intermediates Containing Double Holliday Junctions. We set out to generate a dHJ substrate that could be rapidly prepared in signiﬁcant quantities by annealing four single-stranded DNAs (ssDNAs) (Fig. 1 A and B). To recapitulate physiological aspects of dissolution in which two Holliday junc-

WebJan 1, 2009 · The dissolution of double Holliday junction requires the concerted action of BLM and topoisomerase IIIα (compare lane 17 with lanes 5 and 6). Human RMI1 …

WebA double dissolution is a procedure permitted under the Australian Constitution to resolve deadlocks in the bicameral Parliament of Australia between the House of Representatives … example of hypotonic drinkWebJul 9, 2014 · The dissolvasome complex collapses and unlinks a double Holliday junction DNA double-strand breaks can be repaired by homologous recombination, whose key intermediate is the four-way Holliday junction. During meiosis, Holliday junctions are cut by endonucleases to generate crossovers that are key to the proper segregation of … example of hypothesis with variablesWebJan 2, 2014 · PMID: 24392087 PMCID: PMC3879244 DOI: 10.1371/journal.pone.0083582 Abstract Although Blm and Top3α are known to form a minimal dissolvasome that can uniquely undo a double Holliday junction structure, … example of hypothetical deductive thinkingWebSep 9, 2016 · The second end of the DSB can be captured giving rise to a double-Holliday junction ... In addition to the Sgs1-Top3-Rmi1 complex's role in dissolution of MMS-induced X-structures, the Smc5/6 complex and Esc2 are also important for dissolution of these molecules (Choi et al.2010). example of hysteria in the crucibleWebNov 26, 2024 · BLM interacts with TopIII α, RMI1, RMI2, FANCM, and FANCC, bridging key components required for proper DNA repair to dissolve the double Holiday Junctions, an intermediate formed during HRR after the resection step . This results in the dissolution of the double Holiday Junction, which prevents genetic crossover. example of hypotonic fluidWebMay 25, 2010 · Inter-homologue mitotic junctions in recombinational repair. Bzymek et al. showed that double HJs are intermediates in mitotic DSB repair in diploid cells. Double … brunow rightWebJul 1, 2014 · Double Holliday junctions (dHJS) are important intermediates of homologous recombination. The separate junctions can each be cleaved by DNA structure-selective … example of hypothesis in business research